p-mTOR和p-4EBP1在横纹肌肉瘤组织中的表达及其临床意义

童刚领; 孙晓非; 甄子俊; 林琳

doi:10.3969/j.issn.1000-8179.2011.08.007

摘要: 目的：探讨p-mTOR、 p-4EBP1在横纹肌肉瘤组织中的表达及临床意义，并探讨其相关性。方法：采用免疫组化（SABC法）检测70例横纹肌肉瘤组织p-mTOR、 p-4EBP1的表达，分析其与横纹肌肉瘤患者临床病理特征及预后的关系。结果：横纹肌肉瘤组织中p-mTOR、 p-4EBP1蛋白阳性表达率分别为75.7%和65.7%，均呈正相关（P<0.05）。p-mTOR蛋白表达与其病理亚型相关性有统计学意义（P<0.05）， p-mTOR蛋白阴性组患者3年总生存率、无疾病进展生存率均优于阳性组患者（P=0.001， P=0.014）。p-4EBP1蛋白表达与患者的肿瘤大小的相关性有统计学意义（P<0.05）， p-4EBP1蛋白阴性组患者3年总生存率、无疾病进展生存率均优于阳性组患者（P=0.017， P=0.052）。单因素分析显示， p-mTOR、 p-4EBP1、分期、病理类型、肿瘤大小是不良预后因素（P<0.05），而淋巴结转移情况、性别、年龄与预后无关（P>0.05）。结论：横纹肌肉瘤组织中存在p-mTOR、 p-4EBP1蛋白的高p-mTOR、 p-4EBP1的表达存在相关性， p-mTOR和p-4EBP1表达是横纹肌肉瘤的不良预后因素。

Abstract: Expression of p-mTOR/p-4EBP1 in Rhabdomyosarcoma and Its Clinical SignificanceGangling TONG, Xiaofei SUN, Zijun ZHEN, Lin LINCorrespondence to: Xiaofei SUN, E- mail: gzsunxf@yahoo.com.cnDepartment of Medical Pediatric Oncology, Cancer Center, Sun Yat-sen University, Guangzhou 510060, China.Abstract Objective: To detect the expression of p-mTOR and p-4EBP1 in rhabdomyosarcoma and to explore its clinical signifi-cance. Methods: The expression of p-mTOR and p-4EBP1 in 70 cases of rhabdomyosarcoma was detected by SABC immunohisto-chemistry. The correlation of p-mTOR and p-4EBP1 expression with the clinicopathologic characteristics and prognosis of the rhabdo-myosarcoma were analyzed. Results: The expression rates of p-mTOR and p-4EBP1 in rhabdomyosarcoma were 75.7% and 65.7%, re-spectively. p-mTOR expression was positively correlated with p-4EBP1 expression ( P < 0.05 ). The expression of p-mTOR was signifi-cantly correlated with pathological subtypes ( P < 0.05 ). The 3-year overall survival and disease-free survival of patients withoutp-mTOR expression were higher than those of patients with positive p-mTOR expression ( P = 0.001, P = 0.014 ). The expression ofp-4EBP1 was significantly correlated with the size of rhabdomyosarcoma ( P < 0.05 ). The 3-year overall survival and disease-free sur-vival of patients without p-4EBP1 expression were higher than those of patients with positive p-4EBP1 expression ( P = 0.017, P =0.052 ). Univariate analysis showed that p-mTOR, p-4EBP1, stage, pathological subtypes, and tumor size were negative prognostic fac-tors for patients with RMS ( P < 0.05 ). Nevertheless, lymph node metastasis, sex, and age were not associated with prognosis ( P >0.05). Conclusion: p-mTOR and p-4EBP1 are overexpressed in rhabdomyosarcoma and are correlated with clinicopathologic character-istics of rhabdomyosarcoma. p-mTOR expression is correlated with p-4EBP1 expression in rhabdomyosarcoma. The expression ofp-mTOR and p-4EBP1 is a negative prognostic factor for rhabdomyosarcoma.Keywords Rhabdomyosarcoma; p-mTOR; p-4EBP1; Prognosis